Nerve growth factor induced modification of presynaptic elements in adult visual cortex in vivo
Identifieur interne : 003E26 ( Main/Exploration ); précédent : 003E25; suivant : 003E27Nerve growth factor induced modification of presynaptic elements in adult visual cortex in vivo
Auteurs : Yulin Liu [Canada, États-Unis] ; Karina F. Meiri [États-Unis] ; Max S. Cynader [Canada] ; Qiang Gu [Canada]Source :
- Brain Research [ 0006-8993 ] ; 1996.
English descriptors
Abstract
Nerve growth factor (NGF) has been shown to play important roles in neuronal survival, growth and differentiation. Recently, we have found that intracortical infusion of NGF into adult cat visual cortex can recreate ocular dominance plasticity, suggesting that NGF is also involved in activity-dependent modification of synaptic connectivity in the adult brain. To further explore the mechanisms of NGF-induced plasticity in adult visual cortex, we studied two presynaptic markers: GAP-43 and synaptophysin. Immunocytochemical staining showed that NGF-treatment of adult visual cortex selectively increased the level of the phosphorylated form of GAP-43, while the total level of GAP-43 was not changed. These results demonstrate that NGF-treatment stimulates phosphorylation processes of GAP-43 in vivo. In addition, NGF-treatment of adult visual cortex increased the level of synaptophysin immunoreactivity. Since the phosphorylated form of GAP-43 is known to be enriched in the membrane skeleton of growth cones and of developing synapses, and the phosphorylation of GAP-43 has been linked with events that underlie synaptic plasticity, and since synaptophysin is a major component of presynaptic vesicles, our results suggest that NGF-treatment of adult visual cortex modulates presynaptic terminals, possibly by inducing axonal sprouting and formation of new synapses, and that these changes may play a role in the NGF-induced functional plasticity.
Url:
DOI: 10.1016/0006-8993(96)00484-2
Affiliations:
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Cynader</name></author><author><name sortKey="Gu, Qiang" sort="Gu, Qiang" uniqKey="Gu Q" first="Qiang" last="Gu">Qiang Gu</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:AB04F232B11DB71DED31D97EF9001485EE805A37</idno><date when="1996" year="1996">1996</date><idno type="doi">10.1016/0006-8993(96)00484-2</idno><idno type="url">https://api-v5.istex.fr/document/AB04F232B11DB71DED31D97EF9001485EE805A37/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001D16</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001D16</idno><idno type="wicri:Area/Istex/Curation">001D16</idno><idno type="wicri:Area/Istex/Checkpoint">001836</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001836</idno><idno type="wicri:doubleKey">0006-8993:1996:Liu Y:nerve:growth:factor</idno><idno type="wicri:Area/Main/Merge">004516</idno><idno type="wicri:Area/Main/Curation">003E26</idno><idno type="wicri:Area/Main/Exploration">003E26</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Nerve growth factor induced modification of presynaptic elements in adult visual cortex in vivo</title><author><name sortKey="Liu, Yulin" sort="Liu, Yulin" uniqKey="Liu Y" first="Yulin" last="Liu">Yulin Liu</name><affiliation wicri:level="1"><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Ophthalmology, University of British Columbia, 2550 Willow Street, Vancouver,British Columbia V5Z 3N9</wicri:regionArea><wicri:noRegion>British Columbia V5Z 3N9</wicri:noRegion></affiliation><affiliation wicri:level="2"><country xml:lang="fr" wicri:curation="lc">États-Unis</country><wicri:regionArea>1Present address: Department of Pathology, Allegheny Hospital, Medical College of Pennsylvania, Pittsburgh, PA 15212-4772</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Meiri, Karina F" sort="Meiri, Karina F" uniqKey="Meiri K" first="Karina F." last="Meiri">Karina F. 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Recently, we have found that intracortical infusion of NGF into adult cat visual cortex can recreate ocular dominance plasticity, suggesting that NGF is also involved in activity-dependent modification of synaptic connectivity in the adult brain. To further explore the mechanisms of NGF-induced plasticity in adult visual cortex, we studied two presynaptic markers: GAP-43 and synaptophysin. Immunocytochemical staining showed that NGF-treatment of adult visual cortex selectively increased the level of the phosphorylated form of GAP-43, while the total level of GAP-43 was not changed. These results demonstrate that NGF-treatment stimulates phosphorylation processes of GAP-43 in vivo. In addition, NGF-treatment of adult visual cortex increased the level of synaptophysin immunoreactivity. Since the phosphorylated form of GAP-43 is known to be enriched in the membrane skeleton of growth cones and of developing synapses, and the phosphorylation of GAP-43 has been linked with events that underlie synaptic plasticity, and since synaptophysin is a major component of presynaptic vesicles, our results suggest that NGF-treatment of adult visual cortex modulates presynaptic terminals, possibly by inducing axonal sprouting and formation of new synapses, and that these changes may play a role in the NGF-induced functional plasticity.</div></front></TEI><affiliations><list><country><li>Canada</li><li>États-Unis</li></country><region><li>Pennsylvanie</li><li>État de New York</li></region></list><tree><country name="Canada"><noRegion><name sortKey="Liu, Yulin" sort="Liu, Yulin" uniqKey="Liu Y" first="Yulin" last="Liu">Yulin Liu</name></noRegion><name sortKey="Cynader, Max S" sort="Cynader, Max S" uniqKey="Cynader M" first="Max S." last="Cynader">Max S. Cynader</name><name sortKey="Gu, Qiang" sort="Gu, Qiang" uniqKey="Gu Q" first="Qiang" last="Gu">Qiang Gu</name></country><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Liu, Yulin" sort="Liu, Yulin" uniqKey="Liu Y" first="Yulin" last="Liu">Yulin Liu</name></region><name sortKey="Meiri, Karina F" sort="Meiri, Karina F" uniqKey="Meiri K" first="Karina F." last="Meiri">Karina F. Meiri</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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